論文

査読有り 国際誌
2020年10月

Plant-specific Dof transcription factors VASCULAR-RELATED DOF1 and VASCULAR-RELATED DOF2 regulate vascular cell differentiation and lignin biosynthesis in Arabidopsis

Plant Molecular Biology
  • Vasagi Ramachandran
  • ,
  • Yuki Tobimatsu
  • ,
  • Yamamura Masaomi
  • ,
  • Ryosuke Sano
  • ,
  • Toshiaki Umezawa
  • ,
  • Taku Demura
  • ,
  • Misato Ohtani

104
3
開始ページ
263
終了ページ
281
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s11103-020-01040-9
出版者・発行元
Springer Science and Business Media LLC

KEY MESSAGE: Plant-specific Dof transcription factors VDOF1 and VDOF2 are novel regulators of vascular cell differentiation through the course of a lifetime in Arabidopsis, with shifting their transcriptional target genes. Vascular system is one of critical tissues for vascular plants to transport low-molecular compounds, such as water, minerals, and the photosynthetic product, sucrose. Here, we report the involvement of two Dof transcription factors, named VASCULAR-RELATED DOF1 (VDOF1)/VDOF4.6 and VDOF2/VDOF1.8, in vascular cell differentiation and lignin biosynthesis in Arabidopsis. VDOF genes were expressed in vascular tissues, but the detailed expression sites were partly different between VDOF1 and VDOF2. Vein patterning and lignin analysis of VDOF overexpressors and double mutant vdof1 vdof2 suggested that VDOF1 and VDOF2 would function as negative regulators of vein formation in seedlings, and lignin deposition in inflorescence stems. Interestingly, effects of VDOF overexpression in lignin deposition were different by developmental stages of inflorescence stems, and total lignin contents were increased and decreased in VDOF1 and VDOF2 overexpressors, respectively. RNA-seq analysis of inducible VDOF overexpressors demonstrated that the genes for cell wall biosynthesis, including lignin biosynthetic genes, and the transcription factor genes related to stress response and brassinosteroid signaling were commonly affected by VDOF1 and VDOF2 overexpression. Taken together, we concluded that VDOF1 and VDOF2 are novel regulators of vascular cell differentiation through the course of a lifetime, with shifting their transcriptional target genes: in seedlings, the VDOF genes negatively regulate vein formation, while at reproductive stages, the VDOF proteins target lignin biosynthesis.

リンク情報
DOI
https://doi.org/10.1007/s11103-020-01040-9
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32740898
URL
http://link.springer.com/content/pdf/10.1007/s11103-020-01040-9.pdf
URL
http://link.springer.com/article/10.1007/s11103-020-01040-9/fulltext.html
ID情報
  • DOI : 10.1007/s11103-020-01040-9
  • ISSN : 0167-4412
  • eISSN : 1573-5028
  • PubMed ID : 32740898

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