2014年9月
X-ray Scattering from Immunostimulatory Tetrapod-Shaped DNA in Aqueous Solution To Explore Its Biological Activity-Conformation Relationship
JOURNAL OF PHYSICAL CHEMISTRY B
- 巻
- 118
- 号
- 35
- 開始ページ
- 10373
- 終了ページ
- 10379
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/jp505556r
- 出版者・発行元
- AMER CHEMICAL SOC
We carried out synchrotron X-ray scattering experiments from four DNA supermolecules designed to form tetrapod shapes; these supermolecules had different sequences but identical numbers of total base pairs, and each contained an immunostimulatory CpG motif: We confirmed that the supermolecules did indeed form the expected tetrapod shape. The sample that had the largest radius of gyration (R-g) induced the most cytokine secretion from cultured immune cells. Structural analysis in combination with a rigid tetrapod model and an atomic scale DNA model revealed that the larger R-g can be ascribed to dissociation of the DNA double strands in the central connecting portion of the DNA tetrapod. This finding suggests that the biological activity is related to the ease with which single DNA strands can be formed.
- リンク情報
- ID情報
-
- DOI : 10.1021/jp505556r
- ISSN : 1520-6106
- PubMed ID : 25122085
- Web of Science ID : WOS:000341337500006