2008年6月
Enhanced immunostimulatory activity of oligodeoxynucleotides by Y-shape formation
IMMUNOLOGY
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- 巻
- 124
- 号
- 2
- 開始ページ
- 247
- 終了ページ
- 255
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/j.1365-2567.2007.02762.x
- 出版者・発行元
- WILEY-BLACKWELL
DNA containing unmethylated CpG dinucleotides (CpG DNA) is a potent activator of innate and acquired immune responses. Although the sequence-specific immunostimulatory activity of CpG DNA has been extensively explored, little information is available about the importance of the stereochemical properties of CpG DNA. In this study, Y-shaped oligodeoxynucleotides (Y-ODNs) were prepared using three ODNs with the halves of each ODN being partially complementary to a half of the other two ODNs. Y-ODN induced greater amounts of tumour necrosis factor-alpha and interleukin-6 from RAW264.7 macrophage-like cells than did conventional single-stranded ODN (ssODN) or double-stranded ODN (dsODN). The Y-ODN was less stable in serum than dsODN, but greater amounts of Y-ODN were taken up by macrophage-like cells compared with dsODN. A newly designed Y-ODN containing three potent CpG motifs generated significantly higher levels of cytokines compared with dsODN containing the identical sequences. These results indicate that the Y-shaped form of ODN is a novel, reproducible and reliable approach to enhancing the immunostimulatory activity of ODNs.
- リンク情報
- ID情報
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- DOI : 10.1111/j.1365-2567.2007.02762.x
- ISSN : 0019-2805
- PubMed ID : 18217956
- Web of Science ID : WOS:000255924900011