The success of gene therapy is largely dependent on the development of gene transfer vector systems. So far, various types of gene delivery vectors, both viral and nonviral, have been developed.Among these, synthetic non-viral vectors, such as cationic lipids and cationic polymers, which are electrostatically complexed with plasmid DNA, are particularly suitable for in vivo gene delivery due to their simplicity of preparation and the absence of a specific immune response. Some of these non-viral vectors have shown promise in in-vivo gene delivery settings (Dow et al. 1999
Hood et al. 2002, Sakurai et al. 2003
Yamada et al. 2003).