論文

査読有り
2008年3月

Cloning and characterization of naringenin 8-prenyltransferase, a flavonoid-specific prenyltransferase of Sophora flavescens

PLANT PHYSIOLOGY
  • Kanako Sasaki
  • ,
  • Kouji Mito
  • ,
  • Kazuaki Ohara
  • ,
  • Hirobumi Yamamoto
  • ,
  • Kazufumi Yazaki

146
3
開始ページ
1075
終了ページ
1084
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1104/pp.107.110544
出版者・発行元
AMER SOC PLANT BIOLOGISTS

Prenylated flavonoids are natural compounds that often represent the active components in various medicinal plants and exhibit beneficial effects on human health. Prenylated flavonoids are hybrid products composed of a flavonoid core mainly attached to either 5-carbon (dimethylallyl) or 10-carbon (geranyl) prenyl groups derived from isoprenoid (terpenoid) metabolism, and the prenyl groups are crucial for their biological activity. Prenylation reactions in vivo are crucial coupling processes of two major metabolic pathways, the shikimate-acetate and isoprenoid pathways, in which these reactions are also known as a rate- limiting step. However, none of the genes responsible for the prenylation of flavonoids has been identified despite more than 30 years of research in this field. We have isolated a prenyltransferase gene from Sophora flavescens, SfN8DT-1, responsible for the prenylation of the flavonoid naringenin at the 8-position, which is specific for flavanones and dimethylallyl diphosphate as substrates. Phylogenetic analysis shows that SfN8DT-1 has the same evolutionary origin as prenyltransferases for vitamin E and plastoquinone. The gene expression of SfN8DT-1 is strictly limited to the root bark where prenylated flavonoids are solely accumulated in planta. The ectopic expression of SfN8DT-1 in Arabidopsis thaliana resulted in the formation of prenylated apigenin, quercetin, and kaempferol, as well as 8-prenylnaringenin. SfN8DT-1 represents the first flavonoid-specific prenyltransferase identified in plants and paves the way for the identification and characterization of further genes responsible for the production of this large and important class of secondary metabolites.

リンク情報
DOI
https://doi.org/10.1104/pp.107.110544
J-GLOBAL
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902239184749070
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18218974
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000256412200033&DestApp=WOS_CPL
ID情報
  • DOI : 10.1104/pp.107.110544
  • ISSN : 0032-0889
  • J-Global ID : 200902239184749070
  • PubMed ID : 18218974
  • Web of Science ID : WOS:000256412200033

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