論文

査読有り
2009年

Improved accumulation of ajmalicine and tetrahydroalstonine in Catharanthus cells expressing an ABC transporter

JOURNAL OF PLANT PHYSIOLOGY
  • Barbora Pomahacova
  • ,
  • Jaroslav Dusek
  • ,
  • Jirina Duskova
  • ,
  • Kazufumi Yazaki
  • ,
  • Sittiruk Roytrakul
  • ,
  • Robert Verpoorte

166
13
開始ページ
1405
終了ページ
1412
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jplph.2009.02.015
出版者・発行元
ELSEVIER GMBH, URBAN & FISCHER VERLAG

The biosynthetic pathway of monoterpenoid indole alkaloids in Catharanthus roseus is located throughout various membranes at both the cellular and intercellular levels. ATP-binding cassette (ABC) transporters are known to export vincristine and vinblastine from human cancer cells. It has recently been shown that ABC transporters are also involved in the transport of various monoterpenoid alkaloids in Catharanthus roseus cells. Over-expression of an ABC transporter in this plant might therefore affect the regulation of the alkaloid biosynthetic pathway. CjMDR1, an ABC transporter gene originally isolated from Coptis japonica, was expressed in Catharanthus roseus cell cultures. Cells showing a positive PCR signal of the transgene in both cDNA and genomic DNA samples were subject to transport studies using selected substrates. Unexpectedly, transport of the isoquinoline alkaloid berberine, the main substrate of CjMDR1 transporter in Coptis japonica, was not affected as compared with control and wild-type Catharanthus cells. On the other hand, the endogenous alkaloids ajmalicine and tetrahydroalstonine were accumulated significantly more in Catharanthus roseus cells expressing CjMDR1 in comparison with control tines after feeding these alkaloids. (C) 2009 Elsevier GmbH. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.jplph.2009.02.015
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000269165200007&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.jplph.2009.02.015
  • ISSN : 0176-1617
  • Web of Science ID : WOS:000269165200007

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