論文

査読有り
2010年12月

Oxalate Efflux Transporter from the Brown Rot Fungus Fomitopsis palustris

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
  • Tomoki Watanabe
  • ,
  • Nobukazu Shitan
  • ,
  • Shiro Suzuki
  • ,
  • Toshiaki Umezawa
  • ,
  • Mikio Shimada
  • ,
  • Kazufumi Yazaki
  • ,
  • Takefumi Hattori

76
23
開始ページ
7683
終了ページ
7690
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1128/AEM.00829-10
出版者・発行元
AMER SOC MICROBIOLOGY

An oxalate-fermenting brown rot fungus, Fomitopsis palustris, secretes large amounts of oxalic acid during wood decay. Secretion of oxalic acid is indispensable for the degradation of wood cell walls, but almost nothing is known about the transport mechanism by which oxalic acid is secreted from F. palustris hyphal cells. We characterized the mechanism for oxalate transport using membrane vesicles of F. palustris. Oxalate transport in F. palustris was ATP dependent and was strongly inhibited by several inhibitors, such as valinomycin and NH4+, suggesting the presence of a secondary oxalate transporter in this fungus. We then isolated a cDNA, FpOAR (Fomitopsis palustris oxalic acid resistance), from F. palustris by functional screening of yeast transformants with cDNAs grown on oxalic acid-containing plates. FpOAR is predicted to be a membrane protein that possesses six transmembrane domains but shows no similarity with known oxalate transporters. The yeast transformant possessing FpOAR (FpOAR-transformant) acquired resistance to oxalic acid and contained less oxalate than the control transformant. Biochemical analyses using membrane vesicles of the FpOAR-transformant showed that the oxalate transport property of FpOAR was consistent with that observed in membrane vesicles of F. palustris. The quantity of FpOAR transcripts was correlated with increasing oxalic acid accumulation in the culture medium and was induced when exogenous oxalate was added to the medium. These results strongly suggest that FpOAR plays an important role in wood decay by acting as a secondary transporter responsible for secretion of oxalate by F. palustris.

リンク情報
DOI
https://doi.org/10.1128/AEM.00829-10
J-GLOBAL
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201102273469927900
CiNii Articles
http://ci.nii.ac.jp/naid/120002723134
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/20889782
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000284310500002&DestApp=WOS_CPL
ID情報
  • DOI : 10.1128/AEM.00829-10
  • ISSN : 0099-2240
  • J-Global ID : 201102273469927900
  • CiNii Articles ID : 120002723134
  • PubMed ID : 20889782
  • Web of Science ID : WOS:000284310500002

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