論文

査読有り
2016年7月

Tyrosine Phosphorylation of SGEF Regulates RhoG Activity and Cell Migration

PLOS ONE
  • Yusuke Okuyama
  • ,
  • Kentaro Umeda
  • ,
  • Manabu Negishi
  • ,
  • Hironori Katoh

11
7
開始ページ
1
終了ページ
13
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0159617
出版者・発行元
PUBLIC LIBRARY SCIENCE

SGEF and Ephexin4 are members of the Ephexin subfamily of RhoGEFs that specifically activate the small GTPase RhoG. It is reported that Ephexin1 and Ephexin5, two well-characterized Ephexin subfamily RhoGEFs, are tyrosine-phosphorylated by Src, and that their phosphorylation affect their activities and functions. In this study, we show that SGEF, but not Ephexin4, is tyrosine-phosphorylated by Src. Tyrosine phosphorylation of SGEF suppresses its interaction with RhoG, the elevation of RhoG activity, and SGEF-mediated promotion of cell migration. We identified tyrosine 530 (Y530), which is located within the Dbl homology domain, as a major phosphorylation site of SGEF by Src, and Y530F mutation blocked the inhibitory effect of Src on SGEF. Taken together, these results suggest that the activity of SGEF is negatively regulated by tyrosine phosphorylation of the DH domain.

リンク情報
DOI
https://doi.org/10.1371/journal.pone.0159617
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000380170100056&DestApp=WOS_CPL
ID情報
  • DOI : 10.1371/journal.pone.0159617
  • ISSN : 1932-6203
  • Web of Science ID : WOS:000380170100056

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