論文

査読有り
2009年12月

HNK-1 (HUMAN NATURAL KILLER-1) GLYCO-EPITOPE IS ESSENTIAL FOR NORMAL SPINE MORPHOGENESIS IN DEVELOPING HIPPOCAMPAL NEURONS

NEUROSCIENCE
  • I. Morita
  • ,
  • S. Kakuda
  • ,
  • Y. Takeuchi
  • ,
  • T. Kawasaki
  • ,
  • S. Oka

164
4
開始ページ
1685
終了ページ
1694
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.neuroscience.2009.09.065
出版者・発行元
PERGAMON-ELSEVIER SCIENCE LTD

The human natural killer-1 (HNK-1) glyco-epitope possesses a unique structural feature, a sulfated glucuronic acid attached to lactosamine on the non-reducing termini of glycans. The expression of HNK-1 is temporally and spatially regulated by glucuronyltransferase (GlcAT-P) in the brain. Our previous report showed that mice lacking GlcAT-P almost completely lost HNK-1 expression in the brain and exhibited reduced long-term potentiation (LTP) at hippocampal CA1 synapses. GlcAT-P-deficient mice also showed impaired hippocampus-dependent spatial learning. Although HNK-1 plays an essential role in synaptic plasticity and memory formation, it remains unclear how HNK-1 regulates these functions. In this study, we showed that loss of the HNK-1 epitope resulted in an increase of filopodium-like immature spines and a decrease of mushroom-like mature spines in both the early postnatal mouse hippocampus and cultured hippocampal neurons. However, HNK-1 had no influence on spine density or filopodium formation. Immunofluorescence staining revealed that loss of HNK-1 altered the distribution of postsynaptic proteins such as alpha-amino-3-hydroxy-5-methylisoxazolepropionate (AMPA)-type glutamate receptor subunit GluR2 and PSD-95 from spine heads onto dendritic shafts without affecting synapse formation, resulting in an increase of shaft synapses in cultured GlcATP-deficient neurons. GluR2, a major HNK-1 carrier glycoprotein in postsynaptic density, has the ability to promote spine morphogenesis. Overexpression of GluR2 promoted spine growth in both wild-type and GlcAT-P-deficient neurons, but the increase in GicAT-P-deficient neurons was lower than that in wild-type neurons. This is the first evidence that HNK-1 is a key factor for normal dendritic spine maturation and is involved in the distribution of postsynaptic proteins. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.neuroscience.2009.09.065
CiNii Articles
http://ci.nii.ac.jp/naid/120001741231
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/19796667
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000272327000030&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.neuroscience.2009.09.065
  • ISSN : 0306-4522
  • CiNii Articles ID : 120001741231
  • PubMed ID : 19796667
  • Web of Science ID : WOS:000272327000030

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