論文

2008年11月

An immunohistochemical study of osteopontin in hepatolithiasis

JOURNAL OF HEPATO-BILIARY-PANCREATIC SURGERY
  • Akihiro Nakai
  • ,
  • Motohiro Imano
  • ,
  • Yoshifumi Takeyama
  • ,
  • Hitoshi Shiozaki
  • ,
  • Harumasa Ohyanagi

15
6
開始ページ
615
終了ページ
621
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00534-007-1320-8
出版者・発行元
SPRINGER TOKYO

Mucin glycoprotein from the intrahepatic bile ducts, and from the intramural and extramural glandular epithelia, is thought to contribute to the formation of the matrix, or nucleus, in hepatolithiasis and to contribute to biomineralization systems. Osteopontin (OPN), a noncollagenous acidic bone matrix glycoprotein that possesses calcium-binding properties. To investigate the role of OPN in the genesis of hepatolithiasis, OPN was immunohistochemically studied in the intrahepatic bile ducts, intramural and extramural glands, and in stones.
An immunohistochemical study was performed in 21 human liver specimens with hepatolithiasis, by the EnVision (Dako Japan, Kyoto, Japan) method.
Staining for OPN was strongly positive in the epithelium of stone-laden intrahepatic bile ducts, intramural and extramural glands, and in stones. The stone-laden intrahepatic bile ducts were infiltrated by macrophages showing intense staining for OPN. Sections of the hepatolithiasis, viewed under low magnification, showed a lamellar pattern of OPN immunolabeling, and they showed a reticular pattern under high magnification. In the center of the stones, large nestlike membranous structures were frequently present.
Our result indicates that OPN, an acidic glycoprotein from intrahepatic bile ducts and from intramural and extramural glandular epithelia, seems to be involved in lithiasis, both as a core protein in the early phase, and in the late phase.


リンク情報
DOI
https://doi.org/10.1007/s00534-007-1320-8
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000260691700009&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00534-007-1320-8
  • ISSN : 0944-1166
  • Web of Science ID : WOS:000260691700009

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