論文

2009年3月

Involvement of osteopontin as a core protein in cholesterol gallstone formation

JOURNAL OF HEPATO-BILIARY-PANCREATIC SURGERY
  • Hideaki Ichikawa
  • ,
  • Motohiro Imano
  • ,
  • Yoshifumi Takeyama
  • ,
  • Hitoshi Shiozaki
  • ,
  • Harumasa Ohyanagi

16
2
開始ページ
197
終了ページ
203
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00534-009-0043-4
出版者・発行元
SPRINGER TOKYO

Matrix proteins are considered to be essential for biomineralization and to be important factors in the formation and growth of gallstones. Osteopontin (Opn) is a noncollagenous, acidic bone-matrix glycoprotein, which is sialated and phosphorylated and which has a cell-binding peptide sequence of glycine-arginine-glycine-aspartate-serine (GRGDS). To investigate the role of Opn in cholesterol gallstone formation, we have studied the involvement of Opn in cholesterol gallstone formation in the human gallbladder wall, in the stones, and in the mouse gallbladder using a gallstone experimental model.
Immunohistochemical staining was used in the human gallbladder wall and human gallstones and the determination of mRNA expression by reverse transcriptase-PCR was used in the mouse gallbladder of a gallstone experimental model.
The epithelium of stone-laden gallbladders demonstrated high Opn reactivity, as did the core of the stones. Microscopically detected early stones without macroscopic evidence of lithiasis showed the same immunoreactivity as larger stones. Stone-laden gallbladders were infiltrated by macrophages showing intense Opn expression. In gallstone-forming mice, the expression of Opn mRNA and its protein were significantly increased in the gallbladder wall in the early phase of a lithogenic diet intake, before the initiation of inflammation.
These results suggest that Opn is possibly involved as a core protein in the formation of cholesterol gallstones.


リンク情報
DOI
https://doi.org/10.1007/s00534-009-0043-4
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000264527300016&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00534-009-0043-4
  • ISSN : 0944-1166
  • Web of Science ID : WOS:000264527300016

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