論文

査読有り 国際誌
2021年7月

The impact of culture dimensionality on behavioral epigenetic memory contributing to pluripotent state of iPS cells.

Journal of cellular physiology
  • Naruchit Thanuthanakhun
  • ,
  • Masahiro Kino-Oka
  • ,
  • Suparerk Borwornpinyo
  • ,
  • Yuzuru Ito
  • ,
  • Mee-Hae Kim

236
7
開始ページ
4985
終了ページ
4996
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/jcp.30211

Three-dimensional (3D) culture platforms have been explored to establish physiologically relevant cell culture environment and permit expansion scalability; however, little is known about the mechanisms underlying the regulation of pluripotency of human induced pluripotent stem cells (hiPSCs). This study elucidated epigenetic modifications contributing to pluripotency of hiPSCs in response to 3D culture. Unlike two-dimensional (2D) monolayer cultures, 3D cultured cells aggregated with each other to form ball-like aggregates. 2D cultured cells expressed elevated levels of Rac1 and RhoA; however, Rac1 level was significantly lower while RhoA level was persisted in 3D aggregates. Compared with 2D monolayers, the 3D aggregates also exhibited significantly lower myosin phosphorylation. Histone methylation analysis revealed remarkable H3K4me3 upregulation and H3K27me3 maintenance throughout the duration of 3D culture; in addition, we observed the existence of naïve pluripotency signatures in cells grown in 3D culture. These results demonstrated that hiPSCs adapted to 3D culture through alteration of the Rho-Rho kinase-phospho-myosin pathway, influencing the epigenetic modifications and transcriptional expression of pluripotency-associated factors. These results may help design culture environments for stable and high-quality hiPSCs.

リンク情報
DOI
https://doi.org/10.1002/jcp.30211
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33305410
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85097484834&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85097484834&origin=inward
ID情報
  • DOI : 10.1002/jcp.30211
  • ISSN : 0021-9541
  • eISSN : 1097-4652
  • PubMed ID : 33305410
  • SCOPUS ID : 85097484834

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