論文

査読有り
2014年5月

Lipocalin 2 binds to membrane phosphatidylethanolamine to induce lipid raft movement in a PKA-dependent manner and modulates sperm maturation

DEVELOPMENT
  • Hitomi Watanabe
  • ,
  • Toru Takeo
  • ,
  • Hiromasa Tojo
  • ,
  • Kazuhito Sakoh
  • ,
  • Thorsten Berger
  • ,
  • Naomi Nakagata
  • ,
  • Tak W. Mak
  • ,
  • Gen Kondoh

141
10
開始ページ
2157
終了ページ
2164
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1242/dev.105148
出版者・発行元
COMPANY OF BIOLOGISTS LTD

Mammalian sperm undergo multiple maturation steps after leaving the testis in order to become competent for fertilization, but the molecular mechanisms underlying this process remain unclear. In terms of identifying factors crucial for these processes in vivo, we found that lipocalin 2 (Lcn2), which is known as an innate immune factor inhibiting bacterial and malarial growth, can modulate sperm maturation. Most sperm that migrated to the oviduct of wild-type females underwent lipid raft reorganization and glycosylphosphatidylinositol-anchored protein shedding, which are signatures of sperm maturation, but few did so in Lcn2 null mice. Furthermore, we found that LCN2 binds to membrane phosphatidylethanolamine to reinforce lipid raft reorganization via a PKA-dependent mechanism and promotes sperm to acquire fertility by facilitating cholesterol efflux. These observations imply that mammals possess a mode for sperm maturation in addition to the albumin-mediated pathway.

リンク情報
DOI
https://doi.org/10.1242/dev.105148
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000336126400018&DestApp=WOS_CPL
ID情報
  • DOI : 10.1242/dev.105148
  • ISSN : 0950-1991
  • eISSN : 1477-9129
  • Web of Science ID : WOS:000336126400018

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