2008年9月
The fusing ability of sperm is bestowed by CD9-containing vesicles released from eggs in mice
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- 巻
- 105
- 号
- 35
- 開始ページ
- 12921
- 終了ページ
- 12926
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1073/pnas.0710608105
- 出版者・発行元
- NATL ACAD SCIENCES
Membrane fusion is an essential step in the encounter of two nuclei from sex cells-sperm and egg-in fertilization. However, aside from the involvement of two molecules, CD9 and Izumo, the mechanism of fusion remains unclear. Here, we show that sperm-egg fusion is mediated by vesicles containing CD9 that are released from the egg and interact with sperm. We demonstrate that the CD9(-/-) eggs, which have a defective sperm-fusing ability, have impaired release of CD9-containing vesicles. We investigate the fusion-facilitating activity of CD9-containing vesicles by examining the fusion of sperm to CD9(-/-) eggs with the aid of exogenous CD9-containing vesicles. Moreover, we show, by examining the fusion of sperm to CD9(-/-) eggs, that hamster eggs have a similar fusing ability as mouse eggs. The CD9-containing vesicle release from unfertilized eggs provides insight into the mechanism required for fusion with sperm.
- リンク情報
-
- DOI
- https://doi.org/10.1073/pnas.0710608105
- CiNii Articles
- http://ci.nii.ac.jp/naid/80019741429
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/18728192
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000259343000055&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1073/pnas.0710608105
- ISSN : 0027-8424
- CiNii Articles ID : 80019741429
- PubMed ID : 18728192
- Web of Science ID : WOS:000259343000055