2013年
Production of mouse pups from germline transmission-failed knockout chimeras
Transgenic Research
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- 巻
- 22
- 号
- 1
- 開始ページ
- 195
- 終了ページ
- 200
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s11248-012-9635-x
Occasionally, chimeras do not transmit the gene of interest to pups in gene disruption experiments. However, the risk of failure could be reduced if we could identify embryonic stem (ES)-derived germ cells in the testis. Here, we report the production of pups from three lines of infertile chimeric male mice and the establishment of knockout lines by combining green fluorescent protein-tagged ES cells with intracytoplasmic sperm injection. © 2012 Springer Science+Business Media B.V.
- リンク情報
- ID情報
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- DOI : 10.1007/s11248-012-9635-x
- ISSN : 0962-8819
- PubMed ID : 22826106
- SCOPUS ID : 84871368189