2011年2月
Structural analysis of N-glycans of the planarian Dugesia japonica
FEBS JOURNAL
- ,
- ,
- ,
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- 巻
- 278
- 号
- 3
- 開始ページ
- 452
- 終了ページ
- 460
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/j.1742-4658.2010.07966.x
- 出版者・発行元
- WILEY-BLACKWELL
To investigate the relationship between phylogeny and glycan structures, we analyzed the structure of planarian N-glycans. The planarian Dugesia japonica, a member of the flatworm family, is a lower metazoan. N-glycans were prepared from whole worms by hydrazinolysis, followed by tagging with the fluorophore 2-aminopyridine at their reducing end. The labeled N-glycans were purified, and separated by three HPLC steps. By comparison with standard pyridylaminated N-glycans, it was shown that the N-glycans of planarian include high mannose-type and pauci-mannose-type glycans. However, many of the major N-glycans from planarians have novel structures, as their elution positions did not match those of the standard glycans. The results of mass spectrometry and sugar component analyses indicated that these glycans include methyl mannoses, and that the most probable linkage was 3-O-methylation. Furthermore, the methyl residues on the most abundant glycan may be attached to the non-reducing-end mannose, as the glycans were resistant to alpha-mannosidase digestion. These results indicate that methylated high-mannose-type glycans are the most abundant structure in planarians.
- リンク情報
- ID情報
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- DOI : 10.1111/j.1742-4658.2010.07966.x
- ISSN : 1742-464X
- PubMed ID : 21205195
- Web of Science ID : WOS:000286328400005