A forced air ventilation system for small laboratory animals was developed. The system consists of an air handling unit with air supply and exhaust funs, a rack, hard cage covers with a large diameter air inlet and an outlet, and shoe box cages. Air flow from the supply duct, to the exhaust duct and within the cage were observed. Variations in air flow among cages was minimal. The optimal air exchange rate of the cages in this system was determined to be 60 times per hour based on the results obtained in the present study. At this air exchange rate, air flow at the base of the cages had a velocity of less than 0.09m/sec, which was within the range of recommended values for humans. The observed results show that the system developed is capable of sustaining a laboratory animal microenvironment well in terms of air flow, without too much energy cost.