2016年1月9日
2D41 細胞間張力を感知するαカテニンの力学的分子適応メカニズム
バイオエンジニアリング講演会講演論文集
- ,
- 巻
- 2016
- 号
- 28
- 開始ページ
- "2D41
- 終了ページ
- 1"-"2D41-4"
- 記述言語
- 日本語
- 掲載種別
- 出版者・発行元
- 一般社団法人日本機械学会
The contractile forces in individual cells drive the tissue dynamics, such as morphogenesis and wound healing, and maintain tissue integrity. In these processes, α-catenin molecule plays as a tension sensor at cadherin-based adherens junctions (AJs), accelerating the positive feedback of intercellular tension. Under tension, α-catenin is activated to recruit vinculin, which recruits actin filaments to AJs. Here, we revealed how α-catenin retains its activated state while avoiding unfolding under tension. By using single-molecule force spectroscopy employing atomic force microscopy (AFM), we found that mechanically activated (under approximately 10 pN) α-catenin exhibited higher mechanical stability than a non-activated fragment. In addition, we demonstrated that mechanically activated α-catenin was further reinforced by vinculin binding. Our data suggest that the plastic characteristics of α-catenin, revealed in response to both mechanical and biochemical cues, enable the functional-structural dynamics at the cellular and tissue levels.
- リンク情報
-
- CiNii Articles
- http://ci.nii.ac.jp/naid/110010052192
- CiNii Books
- http://ci.nii.ac.jp/ncid/AA11903175
- ID情報
-
- CiNii Articles ID : 110010052192
- CiNii Books ID : AA11903175