MISC

2010年

Quantitative mapping of aqueous microfluidic temperature with sub-degree resolution using fluorescence lifetime imaging microscopy

LAB ON A CHIP
  • Emmelyn M. Graham
  • ,
  • Kaoru Iwai
  • ,
  • Seiichi Uchiyama
  • ,
  • A. Prasanna de Silva
  • ,
  • Steven W. Magennis
  • ,
  • Anita C. Jones

10
10
開始ページ
1267
終了ページ
1273
記述言語
英語
掲載種別
DOI
10.1039/b924151e
出版者・発行元
ROYAL SOC CHEMISTRY

The use of a water-soluble, thermo-responsive polymer as a highly sensitive fluorescence-lifetime probe of microfluidic temperature is demonstrated. The fluorescence lifetime of poly(N-isopropylacrylamide) labelled with a benzofurazan fluorophore is shown to have a steep dependence on temperature around the polymer phase transition and the photophysical origin of this response is established. The use of this unusual fluorescent probe in conjunction with fluorescence lifetime imaging microscopy (FLIM) enables the spatial variation of temperature in a microfluidic device to be mapped, on the micron scale, with a resolution of less than 0.1 degrees C. This represents an increase in temperature resolution of an order of magnitude over that achieved previously by FLIM of temperature-sensitive dyes.

リンク情報
DOI
https://doi.org/10.1039/b924151e
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000277359600007&DestApp=WOS_CPL
ID情報
  • DOI : 10.1039/b924151e
  • ISSN : 1473-0197
  • Web of Science ID : WOS:000277359600007

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