論文

査読有り 本文へのリンクあり 国際誌
2018年

Overexpression of the transcription factor Rst2 in Schizosaccharomyces pombe indicates growth defect, mitotic defects, and microtubule disorder

Bioscience, Biotechnology and Biochemistry
  • Kouhei Takenaka
  • ,
  • Takuma Tanabe
  • ,
  • Makoto Kawamukai
  • ,
  • Yasuhiro Matsuo

82
2
開始ページ
247
終了ページ
257
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/09168451.2017.1415126

© 2018 Japan society for Bioscience, Biotechnology, and agrochemistry. In Schizosaccharomyces pombe, the transcription factor Rst2 regulates ste11 in meiosis and fbp1 in glucogenesis downstream of the cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKA) pathway. Here, we demonstrate that Rst2 regulates additional cellular events. Overexpressed Rst2 elevated the frequency of oval, bent, branched, septated, and multi-septated cells. Cells showed normal nuclear divisions but exhibited abnormal nuclear organization at low frequency. In oval cells, microtubules were curved but they were rescued by the deletion of mal3. Since growth defect was not rescued by mal3 deletion, we argue that it is regulated independently. Loss of functional Pka1 exaggerated growth defect upon Rst2 overexpression because its downregulation by Pka1 was lost. Overexpression of Rst2 also caused sensitivity to KCl and CaCl2. These findings suggest that, in addition to meiosis and glucogenesis, Rst2 is involved in cellular events such as regulation of cell growth, cell morphology, mitosis progression, microtubules structure, nuclear structure, and stress response.

リンク情報
DOI
https://doi.org/10.1080/09168451.2017.1415126
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29316864
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000425675900010&DestApp=WOS_CPL
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85044193502&origin=inward 本文へのリンクあり
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85044193502&origin=inward
ID情報
  • DOI : 10.1080/09168451.2017.1415126
  • ISSN : 0916-8451
  • eISSN : 1347-6947
  • PubMed ID : 29316864
  • SCOPUS ID : 85044193502
  • Web of Science ID : WOS:000425675900010

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