論文

査読有り 本文へのリンクあり 国際誌
2016年6月9日

Label-free Chemical Imaging of Fungal Spore Walls by Raman Microscopy and Multivariate Curve Resolution Analysis

Scientific Reports
  • Hemanth Noothalapati
  • ,
  • Takahiro Sasaki
  • ,
  • Tomohiro Kaino
  • ,
  • Makoto Kawamukai
  • ,
  • Masahiro Ando
  • ,
  • Hiro O. Hamaguchi
  • ,
  • Tatsuyuki Yamamoto

6
開始ページ
27789
終了ページ
27789
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/srep27789
出版者・発行元
NATURE PUBLISHING GROUP

Fungal cell walls are medically important since they represent a drug target site for antifungal medication. So far there is no method to directly visualize structurally similar cell wall components such as α-glucan, β-glucan and mannan with high specificity, especially in a label-free manner. In this study, we have developed a Raman spectroscopy based molecular imaging method and combined multivariate curve resolution analysis to enable detection and visualization of multiple polysaccharide components simultaneously at the single cell level. Our results show that vegetative cell and ascus walls are made up of both α- and β-glucans while spore wall is exclusively made of α-glucan. Co-localization studies reveal the absence of mannans in ascus wall but are distributed primarily in spores. Such detailed picture is believed to further enhance our understanding of the dynamic spore wall architecture, eventually leading to advancements in drug discovery and development in the near future.

リンク情報
DOI
https://doi.org/10.1038/srep27789
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27278218
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899791
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000377543000001&DestApp=WOS_CPL
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84973875510&origin=inward 本文へのリンクあり
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84973875510&origin=inward
ID情報
  • DOI : 10.1038/srep27789
  • ISSN : 2045-2322
  • eISSN : 2045-2322
  • PubMed ID : 27278218
  • PubMed Central 記事ID : PMC4899791
  • SCOPUS ID : 84973875510
  • Web of Science ID : WOS:000377543000001

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