Papers

Peer-reviewed Last author
Apr, 2019

Collagen XVIII Deposition in the Basement Membrane Zone beneath the Newly Forming Epidermis during Wound Healing in Mice.

Acta medica Okayama
  • Takahiro Maeba
  • ,
  • Tomoko Yonezawa
  • ,
  • Mitsuaki Ono
  • ,
  • Yasuko Tomono
  • ,
  • Ritva Heljasvaara
  • ,
  • Taina Pihlajaniemi
  • ,
  • Kiichi Inagawa
  • ,
  • Toshitaka Oohashi

Volume
73
Number
2
First page
135
Last page
146
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.18926/AMO/56649

The basement membrane (BM) is composed of various extracellular molecules and regulates tissue regeneration and maintenance. Here, we demonstrate that collagen XVIII was spatiotemporally expressed in the BM during skin wound healing in a mouse excisional wound-splinting model. Re-epithelialization was detected at days 3 and 6 post-wounding. The ultrastructure of epidermal BM was discontinuous at day 3, whereas on day 6 a continuous BM was observed in the region proximal to the wound edge. Immunohistochemistry demonstrated that collagen XVIII was deposited in the BM zone beneath newly forming epidermis in day 3 and 6 wounds. Laminin-332, known to be the earliest BM component appearing in wounds, was colocalized with collagen XVIII in the epidermal BM zone at days 3 and 6. The deposition of α1(IV) collagen and nidogen-1 in the epidermal BM zone occurred later than that of collagen XVIII. We also observed the short isoform of collagen XVIII in the epidermal BM zone at day 3 post-wounding. Collectively, our results suggested that collagen XVIII plays a role in the formation of the dermal-epidermal junction during re-epithelialization, and that it is the short isoform that is involved in the early phase of re-epithelialization.

Link information
DOI
https://doi.org/10.18926/AMO/56649
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31015748
ID information
  • DOI : 10.18926/AMO/56649
  • Pubmed ID : 31015748

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