Papers

Peer-reviewed International journal
Jul, 1996

Nephritogenicity and alpha-chain composition of NC1 fractions of type IV collagen from bovine renal basement membrane.

Virchows Archiv : an international journal of pathology
  • S Rauf
  • ,
  • M Kagawa
  • ,
  • Y Kishiro
  • ,
  • S Inoue
  • ,
  • I Naito
  • ,
  • T Oohashi
  • ,
  • M Sugimoto
  • ,
  • Y Ninomiya
  • ,
  • Y Sado

Volume
428
Number
4-5
First page
281
Last page
8
Language
English
Publishing type
Research paper (scientific journal)

Nephritogenicity (anti-GBM-nephritis-inducing activity) and alpha-chain composition of globular-do-main (NCI) fractions of type IV collagen from bovine renal, pulmonary, and placental basement membranes (BMs) was examined by injecting these fractions with adjuvant into WKY/NCrj rats and by Western blotting using epitope-defined monoclonal antibodies to the six different alpha chains of type IV collagen. A purified nephritogenic fraction from renal BM contained alpha 1-alpha 6(IV)NCI, whereas a non-nephritogenic fraction contained only alpha 1-alpha 2(IV)NCI. Renal and pulmonary NCI had strong nephritogenic activity: placental NCI had weak activity. The renal and pulmonary fractions contained alpha 1-alpha 6(IV)NCI, and the placental fraction had a large amount of alpha 1-alpha 2(IV)NCI and a very small amount of alpha 3-alpha 6(IV)NCI. Immunohistochemical study of bovine renal BM with the monoclonal antibodies revealed that bovine glomerular BM contained alpha 1-alpha 5(IV) chains, but not the alpha 6(IV) chain. The absence of alpha 6(IV) chain in glomerular BM in bovine and in humans indicates that alpha 6(IV) chain is not a target antigen of anti-GBM nephritis. Nephritogenicity is apparently a property of alpha 3-alpha 5(IV)NCI.

Link information
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/8764938
ID information
  • ISSN : 0945-6317
  • Pubmed ID : 8764938

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