1999年
L-selectin and its ligands mediate infiltration of mononuclear cells into kidney interstitium after ureteric obstruction
Journal of Pathology
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- 巻
- 188
- 号
- 1
- 開始ページ
- 93
- 終了ページ
- 99
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1002/(SICI)1096-9896(199905)188:1<93::AID-PATH305>3.0.CO;2-#
It was previously reported that the L-selectin ligands detected by a rat L-selectin and human IgG chimeric molecule (rLEC-IgG) are expressed in the distal tubules of the kidney, where no leukocyte traffic is seen under physiological conditions. In the present study, the role of L-selectin ligands in leukocyte infiltration into the kidney interstitium was investigated using a rat ureteric obstruction model. After ligation of the ureter, ligands for L-selectin rapidly disappeared from tubular epithelial cells and were relocated to the interstitium and peritubular capillary walls, where infiltration of monocytes and CD8+ T cells subsequently occurred. Mononuclear cell infiltration was significantly inhibited by intravenous injection of a neutralizing monoclonal antibody (MAb) against L-selectin, indicating the possible involvement of an L-selectin-mediated pathway. Interestingly, immunohistochemical studies with a MAb against sulphatide showed that the distribution of sulphatide, known to be one of the candidates of L-selectin ligand, was almost indistinguishable from the staining pattern of rLEC-IgG in both normal and ureteric obstructed kidneys, suggesting that sulphatide and/or related molecule(s) relocated to the renal interstitium were recognized by leukocyte L-selectin, leading to interstitial leukocyte infiltration. In line with this notion, intravenous injection of sulphatide markedly inhibited leukocyte infiltration, suggesting that L-selectin- sulphatide interaction may play a pivotal role in interstitial leukocyte infiltration in the kidney following ureteric obstruction.
- リンク情報
- ID情報
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- DOI : 10.1002/(SICI)1096-9896(199905)188:1<93::AID-PATH305>3.0.CO;2-#
- ISSN : 0022-3417
- PubMed ID : 10398147
- SCOPUS ID : 0032916847