Papers

International journal
Jun 15, 2006

Efficacy of atelocollagen honeycomb scaffold in bone formation using KUSA/A1 cells

Journal of Biomedical Materials Research - Part A
  • Andrea Paola Rodriguez
  • ,
  • Liliana Missana
  • ,
  • Hitoshi Nagatsuka
  • ,
  • Mehmet Gunduz
  • ,
  • Hidetsugu Tsujigiwa
  • ,
  • Rosario Rivera
  • ,
  • Noriyuki Nagai

Volume
77
Number
4
First page
707
Last page
717
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1002/jbm.a.30645
Publisher
WILEY-LISS

To induce new bone formation, mesenchymal stem cells were seeded onto atelocollagen honeycomb scaffold. We evaluated the efficacy of this scaffold combined with KUSA/A1 cells in vivo. KUSA/A1 cells alone and with atelocollagen were implanted in the subcutaneous pockets of 4-week-old male SCID mice. The transplants were subjected to radiographical, histological, and immunohistochemical examinations after 2 and 4 weeks of implantation. Radiographically, both KUSA/A1 cells alone and KUSA/A1-atelocollagen showed some radiopaque areas formation but the latter disclosed a larger amount. Histologically, KUSA/A1 cells alone showed few small islands of new bone formation surrounded by a thin layer of cellular proliferation. On the other hand, KUSA/A1-atelocollagen revealed abundant new bone formation as well as cellular proliferation. We also determined the immunolocalization of type I collagen, CD34, osteocalcin, osteopontin, and PCNA in this newly formed bone. Our results indicated that collagen scaffold plays an important role allowing vessel formation and cell anchorage, especially through the proliferation and differentiation process in a confined space. This study supports tissue engineering as an alternative for treating different target diseases, such as trauma or congenital defects, and enhances existing therapeutic applications. © 2006 Wiley Periodicals, Inc.

Link information
DOI
https://doi.org/10.1002/jbm.a.30645
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/16555268
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000237792300007&DestApp=WOS_CPL
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34247199841&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=34247199841&origin=inward
ID information
  • DOI : 10.1002/jbm.a.30645
  • ISSN : 1549-3296
  • eISSN : 1552-4965
  • Pubmed ID : 16555268
  • SCOPUS ID : 34247199841
  • Web of Science ID : WOS:000237792300007

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