Papers

International journal
Jan, 2009

Expression and characterization of PGD<inf>2</inf> receptors in chronic rhinosinusitis: Modulation of DP and CRTH2 by PGD<inf>2</inf>

International Archives of Allergy and Immunology
  • Miki Yamamoto
  • Mitsuhiro Okano
  • Tazuko Fujiwara
  • Shin Kariya
  • Takaya Higaki
  • Hitoshi Nagatsuka
  • Hidetsugu Tsujigiwa
  • Masao Yamada
  • Tadashi Yoshino
  • Yoshihiro Urade
  • Kazunori Nishizaki
  • Display all

Volume
148
Number
2
First page
127
Last page
136
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1159/000155743
Publisher
KARGER

Background: Prostaglandin D2 (PGD2) participates in airway inflammation. We reported that levels of hematopoietic-type PGD 2 synthase (h-PGDS) in sinonasal tissues may play an important role in the pathophysiology of chronic rhinosinusitis (CRS). Two PGD2 receptors have been isolated, DP and CRTH2, but whether they participate in CRS remains unclear. We sought to determine the expression and characterization of DP and CRTH2 in CRS. Methods: Expression of DP and CRTH2 in nasal polyps (NP) and uncinate process mucosae (UPM) was examined by in situ hybridization and immunohistochemistry and evaluated by real-time quantitative PCR. h-PGDS, IL-5, eotaxin and RANTES expression was also determined. In addition, the effect of PGD2 on the expression of both receptors in UPM was assessed. Results: DP was widely expressed, not only in infiltrating inflammatory cells but also in constitutive cells such as vascular endothelial cells and ciliated columnar epithelia. CRTH2 was selectively expressed in inflammatory cells and some glands. Significantly greater levels of DP mRNA and conversely decreased levels of CRTH2 mRNA were observed in NP compared with UPM. DP and CRTH2 mRNA levels were not only positively and inversely correlated with levels of h-PGDS but also with eotaxin, respectively. Furthermore, addition of PGD2 significantly increased DP expression and conversely reduced CRTH2 expression in UPM. Conclusions: These results suggest that distinct expression of DP and CRTH2 is associated with the pathophysiology of CRS, including NP formation, and the expression of these receptors may be regulated by h-PGDS and PGD 2. Copyright © 2008 S. Karger AG.

Link information
DOI
https://doi.org/10.1159/000155743
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18802357
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000261830300005&DestApp=WOS_CPL
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=51849118062&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=51849118062&origin=inward
ID information
  • DOI : 10.1159/000155743
  • ISSN : 1018-2438
  • eISSN : 1423-0097
  • Pubmed ID : 18802357
  • SCOPUS ID : 51849118062
  • Web of Science ID : WOS:000261830300005

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