2009
Establishment of a lymph node metastasis model from subcutaneous tumors of gastrointestinal stromal tumor model cells
Oncology Reports
- Volume
- 21
- Number
- 2
- First page
- 407
- Last page
- 411
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.3892/or_00000237
- Publisher
- SPANDIDOS PUBL LTD
There is currently no suitable animal model of metastasis using cultured human gastrointestinal stromal tumor cells even though the molecular mechanisms of c-KIT-mediated progression and metastasis should be clarified. Ba/F3 murine lymphocyte cells transduced with mutant c-KIT have been utilized to analyze some molecular mechanisms related to a constitutively activated c-KIT signaling and to assess the efficacy of molecular-targeted inhibitors. Using this cellular system, we coincidentally discovered the development of axillary and inguinal lymph node swelling three weeks after subcutaneous injection of Ba/F3 cells with c-KIT mutation into nude mice. Mutation-specific PCR detected c-KIT mutation in the swollen lymph nodes but not in unmetastasized normal lymph nodes, indicating that the lymph nodes contain tumor cells which should come from a primary subcutaneous tumor. Microscopic observation revealed tumor cells infiltrating through lymphatic follicles with Ki-67-positive staining to distinguish them from lymphocytes. The significance of this model is helpful to understand the molecular mechanisms of c-KIT-mediated metastasis and is useful for assessments of molecular therapeutics and in vivo imaging.
- Link information
-
- DOI
- https://doi.org/10.3892/or_00000237
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/19148515
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000262679300020&DestApp=WOS_CPL
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=62849106348&origin=inward Open access
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=62849106348&origin=inward
- ID information
-
- DOI : 10.3892/or_00000237
- ISSN : 1021-335X
- eISSN : 1791-2431
- Pubmed ID : 19148515
- SCOPUS ID : 62849106348
- Web of Science ID : WOS:000262679300020