1999年
Expression characteristics of CS-ACS1, CS-ACS2 and CS-ACS3, three members of the 1-aminocyclopropane-1-carboxylate synthase gene family in cucumber (Cucumis sativus L.) fruit under carbon dioxide stress
Plant and Cell Physiology
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- 巻
- 40
- 号
- 2
- 開始ページ
- 164
- 終了ページ
- 172
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1093/oxfordjournals.pcp.a029524
- 出版者・発行元
- Japanese Society of Plant Physiologists
We investigated the expression pattern of three 1-aminocyclopropane-1-carboxylate (ACC) synthase genes, CS-ACS1, CS-ACS2 and CS-ACS3 in cucumber (Cucumis sativus L.) fruit under CO2 stress. CO2 stress-induced ethylene production paralleled the accumulation of only CSACS1 transcripts which disappeared upon withdrawal of CO2. Cycloheximide inhibited the CO2 stress-induced ethylene production but superinduced the accumulation of CS-ACS1 transcript. At higher concentrations, cycloheximide also induced the accumulation of CS-ACS2 and CS-ACS3 transcripts. In the presence of CO2 and cycloheximide, the accumulation of CS-ACS2 transcript occurred within 1 h, disappeared after 3 h and increased greatly upon withdrawal of CO2. Inhibitors of protein kinase and types 1 and 2A protein phosphatases which inhibited and stimulated, respectively, CO2 stress-induced ethylene production had little effect on the expression of these genes. The results presented here identify CS-ACS1 as the main ACC synthase gene responsible for the increased ethylene biosynthesis in cucumber fruit under CO2 stress and suggest that this gene is a primary response gene and its expression is under negative control since it is expressed by treatment with cycloheximide. The results further suggest that the regulation of CO2 stress-induced ethylene biosynthesis by reversible protein phosphorylation does not result from enhanced ACC synthase transcription.
- リンク情報
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- DOI
- https://doi.org/10.1093/oxfordjournals.pcp.a029524
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/10202812
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0033083026&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=0033083026&origin=inward
- ID情報
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- DOI : 10.1093/oxfordjournals.pcp.a029524
- ISSN : 0032-0781
- PubMed ID : 10202812
- SCOPUS ID : 0033083026