論文

査読有り 国際誌
2020年10月2日

High-performance liquid chromatographic profile and 1H quantitative nuclear magnetic resonance analyses for quality control of a Xinjiang licorice extract

Bioscience, Biotechnology, and Biochemistry
  • Atsumi Miyazaki
  • ,
  • Eerdunbayaer
  • ,
  • Tsugumi Shiokawa
  • ,
  • Hiroko Tada
  • ,
  • Yunhe Lian
  • ,
  • Shoko Taniguchi
  • ,
  • Tsutomu Hatano

84
10
開始ページ
2128
終了ページ
2138
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/09168451.2020.1785272
出版者・発行元
Oxford University Press (OUP)

<title>Abstract</title>
Various pharmacological properties of Xinjiang licorice flavonoids have been reported recently. We have investigated constituents corresponding to distinct peaks on the high-performance liquid chromatography (HPLC) profile of a flavonoid-rich extract from licorice, and identified 13 flavonoids, including licochalcone A (1), licochalcone B (3), glabrone (4), and echinatin (5), by isolating them and then performing high-resolution electrospray ionization mass spectrometry and 1H nuclear magnetic resonance (NMR) spectral analyses. We then applied the 1H quantitative NMR (qNMR) method for analysis of major flavonoids, 1 and 3–5 in the extract. The 1H qNMR results were supported by 13C NMR analysis. The results demonstrated the utility of the combination of HPLC profiling and qNMR analyses for quality control of Xinjiang licorice. Additionally, we observed a moderate inhibitory effect of the most abundant constituent, licochalcone A (1), on acetylcholine esterase activity, suggesting utility as a seed for drug development.

リンク情報
DOI
https://doi.org/10.1080/09168451.2020.1785272
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32614708
URL
http://academic.oup.com/bbb/article-pdf/84/10/2128/36826904/bbb2128.pdf
ID情報
  • DOI : 10.1080/09168451.2020.1785272
  • ISSN : 0916-8451
  • eISSN : 1347-6947
  • PubMed ID : 32614708

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