MISC

2004年5月

Aryl hydrocarbon receptor-mediated induction of microsomal drug-metabolizing enzyme activity by indirubin and indigo

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • K Sugihara
  • S Kitamura
  • T Yamada
  • T Okayama
  • S Ohta
  • K Yamashita
  • M Yasuda
  • Y Fujii-Kuriyama
  • K Saeki
  • S Matsui
  • T Matsuda
  • 全て表示

318
2
開始ページ
571
終了ページ
578
記述言語
英語
掲載種別
DOI
10.1016/j.bbrc.2004.04.066
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Indirubin and indigo. which are thought to be natural ligands for aryl hydrocarbon receptor (AhR), showed marked AhR ligand activities in a reporter gene assay using recombinant yeast. Their activities were comparable with or more potent than that of 2,3,7,8-tetrachlorodibenzo-p-dioxin. When indirubin and indigo were administered to mice, ethoxyresorufin-O-dealkylase and methoxyresorufin-O-dealkylase activities in the liver were increased, but subsequently decreased within 2 days. Indirubin was more potent than indigo. Levels of cytochrome P450 1A1/2 proteins and mRNAs in the liver of mice dosed with indirubin were also enhanced. These enhancing effects of indirubin and indigo were not observed in AhR knock-out mice. Ethoxyresorufin-O-dealkylase and methoxyresorufin-O-dealkylase activities in rat hepatocytes and HepG2 cells were enhanced by the addition of indirubin or indigo, but less potently than by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Indigocarmine, a sulfate derivative of indigo, which is used as food additive. did not show these inducing effects on drug-metabolizing enzymes. Our results suggest that indirubin and indigo act as inducers for cytochrome P450 1A1/2 mediated by AhR in mammals in vivo. (C) 2004 Elsevier Inc. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2004.04.066
CiNii Articles
http://ci.nii.ac.jp/naid/80016688111
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/15120638
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000221315500035&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.bbrc.2004.04.066
  • ISSN : 0006-291X
  • CiNii Articles ID : 80016688111
  • PubMed ID : 15120638
  • Web of Science ID : WOS:000221315500035

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