2004年5月
Aryl hydrocarbon receptor-mediated induction of microsomal drug-metabolizing enzyme activity by indirubin and indigo
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- 巻
- 318
- 号
- 2
- 開始ページ
- 571
- 終了ページ
- 578
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/j.bbrc.2004.04.066
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
Indirubin and indigo. which are thought to be natural ligands for aryl hydrocarbon receptor (AhR), showed marked AhR ligand activities in a reporter gene assay using recombinant yeast. Their activities were comparable with or more potent than that of 2,3,7,8-tetrachlorodibenzo-p-dioxin. When indirubin and indigo were administered to mice, ethoxyresorufin-O-dealkylase and methoxyresorufin-O-dealkylase activities in the liver were increased, but subsequently decreased within 2 days. Indirubin was more potent than indigo. Levels of cytochrome P450 1A1/2 proteins and mRNAs in the liver of mice dosed with indirubin were also enhanced. These enhancing effects of indirubin and indigo were not observed in AhR knock-out mice. Ethoxyresorufin-O-dealkylase and methoxyresorufin-O-dealkylase activities in rat hepatocytes and HepG2 cells were enhanced by the addition of indirubin or indigo, but less potently than by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Indigocarmine, a sulfate derivative of indigo, which is used as food additive. did not show these inducing effects on drug-metabolizing enzymes. Our results suggest that indirubin and indigo act as inducers for cytochrome P450 1A1/2 mediated by AhR in mammals in vivo. (C) 2004 Elsevier Inc. All rights reserved.
- リンク情報
-
- DOI
- https://doi.org/10.1016/j.bbrc.2004.04.066
- CiNii Articles
- http://ci.nii.ac.jp/naid/80016688111
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/15120638
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000221315500035&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1016/j.bbrc.2004.04.066
- ISSN : 0006-291X
- CiNii Articles ID : 80016688111
- PubMed ID : 15120638
- Web of Science ID : WOS:000221315500035