2002年9月
Involvement of the leader sequence in Sendai virus pathogenesis revealed by recovery of a pathogenic field isolate from cDNA
JOURNAL OF VIROLOGY
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- 巻
- 76
- 号
- 17
- 開始ページ
- 8540
- 終了ページ
- 8547
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1128/JVI.76.17.8540-8547.2002
- 出版者・発行元
- AMER SOC MICROBIOLOGY
We previously demonstrated that a systematic passage of a pathogenic field isolate of Sendai virus (SeV), the Hamamatsu strain, in embryonated eggs caused attenuation of virulence to mice, and we isolated viral clones of distinct virulence (K. Kiyotani et al. Arch. Virol. 146:893-908, 2001). One of the clones, E15cl2, which was obtained from the virus at the 15th egg passage of E0, the parental Hamamatsu clone for egg passage, had 165-fold-attenuated virulence to mice and possessed only four mutations in the entire 15,384-base genome: in an antigenomic sense, U to A at position 20 (U(20)A) and U to A at position 24 (U(24)A) in the leader sequence, the promoter for transcription and replication, and A to G at position 9346 (silent) and A to U at position 12174 (Ser to Cys) in the L gene. To examine the possibility that leader mutations affect virus pathogenesis; we recovered live viruses from cDNA derived from the Hamamatsu strain. A mutant virus possessing either a mutation of U(20)A or U(24)A in the leader sequence showed a slightly lower pathogenicity than that of the parental virus, whereas a double mutant virus possessing both of the mutations showed 25-fold-attenuated virulence, accompanying a significantly lower virus replication in the mouse lung. Replications of the leader mutant viruses were also impaired in a primary culture of mouse pulmonary epithelial cells but not in chicken embryo fibroblasts. These findings suggest that leader mutations of SeV affect virus pathogenesis by altering virus replication in a host-dependent manner.
- リンク情報
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- DOI
- https://doi.org/10.1128/JVI.76.17.8540-8547.2002
- CiNii Articles
- http://ci.nii.ac.jp/naid/80015478773
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/12163573
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000177334900004&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1128/JVI.76.17.8540-8547.2002
- ISSN : 0022-538X
- CiNii Articles ID : 80015478773
- PubMed ID : 12163573
- Web of Science ID : WOS:000177334900004