MISC

2003年9月

Masking of the contribution of V protein to Sendai virus pathogenesis in an infection model with a highly virulent field isolate

VIROLOGY
  • T Sakaguchi
  • ,
  • K Kiyotani
  • ,
  • H Watanabe
  • ,
  • C Huang
  • ,
  • N Fukuhara
  • ,
  • Y Fujii
  • ,
  • Y Shimazu
  • ,
  • F Sugahara
  • ,
  • Y Nagai
  • ,
  • T Yoshida

313
2
開始ページ
581
終了ページ
587
記述言語
英語
掲載種別
DOI
10.1016/S0042-6822(03)00350-7
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Sendai virus V protein is not essential for virus replication in cultured cells but is essential for efficient virus replication and pathogenesis in mice, indicating that the V protein has a luxury function to facilitate virus propagation in mice. This was discovered in the Z strain, an egg-adapted avirulent laboratory strain. In the present study, we reexamined the function of Sendai virus V protein by generating a V-knockout Sendai virus derived from the Hamamatsu strain, a virulent field isolate, which is an appropriate model for studying the natural course of Sendai virus infection in mice. We unexpectedly found that the V-knockout virus propagated efficiently in mice and was as virulent as the wild-type virus. Switching of the functionally important V unique region demonstrated that this region of the Hamamatsu strain was also functional in a Z strain background. It thus appears that the V protein is nonsense in a field isolate of Sendai virus. However, the V protein was required for virus growth and pathogenesis of the Hamamatsu strain in mice when the virulence of the virus was attenuated by introducing mutations that had been found in an egg-adapted, avirulent virus. The V protein therefore seems to be potentially functional in the highly virulent Hamamatsu strain and to be prominent if virus replication is restricted. (C) 2003 Elsevier Science (USA). All rights reserved.

Web of Science ® 被引用回数 : 4

リンク情報
DOI
https://doi.org/10.1016/S0042-6822(03)00350-7
CiNii Articles
http://ci.nii.ac.jp/naid/80016202460
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/12954223
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000185261400022&DestApp=WOS_CPL

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