J-GLOBAL         Last updated: Mar 6, 2018 at 02:56
Yamaguchi University
Biomolecular Engineering Graduate School of Medicine Applied Molecular Bioscience(Engineering) Graduate School of Medicine(Engineering) YAMAGUCHI UNIVERSITY
Job title
Doctor of Engineering, Master of Science
Other affiliation
Yamaguchi University
Research funding number

Research Areas


Academic & Professional Experience

Feb 2010
May 2010
Graduate School of Medicine(Engineering), YAMAGUCHI UNIVERSITY
May 2010
Apr 2016
Professor, Graduate School of Medicine(Engineering), YAMAGUCHI UNIVERSITY


Mar 1983
Zoological Institute, Faculty of Science, Hiroshima University
Mar 1988
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University

Published Papers

Relationships between cell cycle and conjugation in the ciliate Euplotes patella syngen 2
R. Akada
Arch. Protistenk   129 171-177   1985   [Refereed]
Role of soluble factors (gamones) and calcium ions in conjugaiton of the ciliate Euplotes patella syngen 2
R. Akada
J. Exp. Zool.   233 169-173   1985   [Refereed]
Mating types and mating-inducing factors (gamones) in the ciliate Euplotes patella syngen 2.
R. Akada
Genet. Res.   46 125-132   1985   [Refereed]
Spontaneous selfing (intraclonal conjugation) in the ciliate Euplotes patella syngen 2 takes place without a change in mating type expression.
R. Akada
J. Protozool.   33 235-237   1986   [Refereed]
Partial characterization of the mating-inducing factors (gamones) produced by mating type VI cells in the ciliate Euplotes patella syngen 2.
R. Akada
J. Exp. Zool.   237 287-290   1986   [Refereed]


Genomic analyses of thermotolerant microorganisms used for high-temperature fermentations

Books etc

Minimum GC-rich sequences for overlap extension PCR and primer annealing
Mikiko Nakamura, Ayako Suzuki, Hisashi Hoshida, and Rinji Akada (Part:Contributor)
Hamana press   2014   
DNA Cloning and Assembly Methods

Teaching Experience



Screening of drugs acting on signal transduction pathway
The Others   
Development of novel sake drinks by breeding between natural yeast strains and Kyokai sake yeast strains
The Others   


Apr 1997
Yeast produces ethanol of Japanese Sake and Beer. Good yeast produces good alcoholic drinks. We developed a new method to manipulate yeast genes and constructed genetically modified yeast strains that produce good sake. Our yeast is called self-cloning yeast which is applicable for commercial Sake production. Our yeast is not reffered as GMO (genetically modified organism) because no extraneous genes are present in the yeast.
Apr 2005
Yeast is useful for drug screeing. Yeast gene manipulation is easy, thus we can construct many yeast strains that are sensitive to specific drugs. This type of yeast strains can be used for drug screening. We found several anticancer drugs from yeast screening.
Apr 2006
The thermotolerant yeast Kluyveromyces marxianus can grow and ferment at high temperatures. In addition, this yeast has efficient non-homologous end joining system. This yeast is used for the development of new genetic engineering called 'linear DNA technology'.
Apr 2006
The thermotolerant yeast can produce ethanol efficiently. The optimum temperature for ethanol fermentation is 40 degree, and even at 45 degree, this yeast can produce ethanol. The ability is similar to the conventional yeast but the high-temperature fermentation is special feature of this yeast. Ethanol production from molasses, cassava, or cellulosic materials will be achieved by this yeast.
Apr 2009
New gene manipulation methods for mammalian culture cells are developed. Usually, gene analysis is performed by using E. coli plasmids but we developed new methods that use PCR-amplified DNA for mammalian gene expression. We applied these methods for the analysis of iPS factors and others.