MISC

2005年2月

Cyclization mechanism for the synthesis of macrocyclic antibiotic lankacidin in Streptomyces rochei

CHEMISTRY & BIOLOGY
  • K Arakawa
  • ,
  • F Sugino
  • ,
  • K Kodama
  • ,
  • T Ishii
  • ,
  • H Kinashi

12
2
開始ページ
249
終了ページ
256
記述言語
英語
掲載種別
DOI
10.1016/j.chembiol.2005.01.009
出版者・発行元
CELL PRESS

The lankacidin biosynthetic gene cluster in Streptomyces rochei strain 7434AN4 was found to span 31 kb of the giant linear plasmid pSLA2-L and contain a polyketide synthase (PKS)/nonribosomal peptide synthetase (NRPS) hybrid gene (IkcA), type I PKS genes, and pyrroloquinoline quinone (PQQ) biosynthetic genes (IkcK-IkcO). Feeding of PQQ to a pqq mutant restored the lankacidin production, suggesting its crucial role in an oxidation process. However, formation of the 17-membered macrocyclic ring was not catalyzed by PQQ-dependent dehydrogenase (Orf23), but was by flavin-dependent amine oxidase (LkcE). Compound LC-KA05 isolated from an IkcE disruptant was an acyclic intermediate lacking the C2-C18 linkage. These results suggested a cyclization mechanism for the synthesis of the lankacidin macrocyclic skeleton.

Web of Science ® 被引用回数 : 64

リンク情報
DOI
https://doi.org/10.1016/j.chembiol.2005.01.009
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000227417300014&DestApp=WOS_CPL