MISC

2004年10月

Two chimeric chromosomes of Streptomyces coelicolor A3(2) generated by single crossover of the wild-type chromosome and linear plasmid SCP1

JOURNAL OF BACTERIOLOGY
  • M Yamasaki
  • ,
  • H Kinashi

186
19
開始ページ
6553
終了ページ
6559
記述言語
英語
掲載種別
DOI
10.1128/JB.186.19.6553-6559.2004
出版者・発行元
AMER SOC MICROBIOLOGY

Streptomyces coelicolor A3(2) strain 2106 carries a 1.85-Mb linear plasmid, SCP1'-cysD, in addition to a 7.2-Mb linear chromosome. Macrorestriction analysis indicated that both linear DNAs are hybrids of the wild-type chromosome and the linear plasmid SCP1 on each side. Nucleotide sequencing of the fusion junctions revealed no homology between the recombination regions. SCP1'-cysD contains an SCP1 telomere and a chromosomal telomere at each end and therefore does not have terminal inverted repeats. In addition, SCP1'-cysD could not be eliminated from strain 2106 by various mutagenic treatments. Thus, we concluded that both the 7.2-Mb chromosome and SCP1'-cysD are chimeric chromosomes generated by a single crossover of the wild-type chromosome and SCP1. This may be regarded as a model of chromosomal duplication in genome evolution.

Web of Science ® 被引用回数 : 21

リンク情報
DOI
https://doi.org/10.1128/JB.186.19.6553-6559.2004
CiNii Articles
http://ci.nii.ac.jp/naid/80016958180
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/15375137
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000223988700025&DestApp=WOS_CPL