論文

査読有り 国際誌
2018年11月

Full genome sequence of a polyvalent bacteriophage infecting strains of Shigella, Salmonella, and Escherichia.

Archives of virology
  • Alaaeldin Mohamed Saad
  • ,
  • Ahmed Askora
  • ,
  • Ahmed Mahrous Soliman
  • ,
  • Hirofumi Nariya
  • ,
  • Takeru Kawasaki
  • ,
  • Makoto Fujie
  • ,
  • Tadashi Shimamoto
  • ,
  • Takashi Yamada

163
11
開始ページ
3207
終了ページ
3210
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00705-018-3971-y

A novel lytic bacteriophage, Escherichia phage EcS1, was isolated from sewage samples collected in Higashi-Hiroshima, Japan. The complete genome sequence of EcS1 was determined using the Illumina Miseq System. The whole genome of EcS1 was found to be 175,437 bp in length with a mean G+C content of 37.8%. A total of 295 genes were identified as structural, functional, and hypothetical genes. BLAST analysis of the EcS1 genomic sequence revealed the highest identity (79%; query cover of 73-74%) to three T4-related phages that infect Serratia sp. ATCC 39006. Host range experiments revealed that EcS1 has lytic effects on three pathogenic strains of Shigella spp. and a pathogenic strain of Salmonella enterica as well as on E. coli strains. However, two strains of Serratia marcescens showed resistance to this phage. Phylogenetic trees for phage tail fiber protein sequences revealed that EcS1 is closely related to Enterobacteriaceae-infecting phages. Thus, EcS1 is a novel phage that infects several pathogenic strains of the family Enterobacteriaceae.

リンク情報
DOI
https://doi.org/10.1007/s00705-018-3971-y
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30056554
ID情報
  • DOI : 10.1007/s00705-018-3971-y
  • PubMed ID : 30056554

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