論文

査読有り 責任著者
2020年8月25日

3D structure of three jumbo phage heads

Journal of General Virology
  • Emmanuelle Neumann
  • ,
  • Takeru Kawasaki
  • ,
  • Grégory Effantin
  • ,
  • Leandro F. Estrozi
  • ,
  • Orawan Chatchawankanphanich
  • ,
  • Takashi Yamada
  • ,
  • Guy Schoehn

101
11
開始ページ
1487-1
終了ページ
1487-8
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1099/jgv.0.001487
出版者・発行元
Microbiology Society

Jumbo phages are bacteriophages that carry more than 200 kbp of DNA. In this study we characterized two jumbo phages (ΦRSL2 and ΦXacN1) and one semi-jumbo phage (ΦRP13) at the structural level by cryo-electron microscopy. Focusing on their capsids, three-dimensional structures of the heads at resolutions ranging from 16 to 9 Å were calculated. Based on these structures we determined the geometrical basis on which the icosahedral capsids of these phages are constructed, which includes the accessory and decorative proteins that complement them. A triangulation number novel to <italic>Myoviridae</italic> (ΦRP13; <italic>T</italic>=21) was discovered as well as two others, which are more common for jumbo phages (<italic>T</italic>=27 and <italic>T</italic>=28). Based on one of the structures we also provide evidence that accessory or decorative proteins are not a prerequisite for maintaining the structural integrity of very large capsids.

リンク情報
DOI
https://doi.org/10.1099/jgv.0.001487
URL
https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001487?crawler=true
ID情報
  • DOI : 10.1099/jgv.0.001487
  • ISSN : 0022-1317
  • eISSN : 1465-2099

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