論文

査読有り
2015年3月

The effect of estrogen on the early cytotoxic response to IB virus infection in hen oviduct

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
  • Takahiro Nii
  • ,
  • Naoki Isobe
  • ,
  • Yukinori Yoshimura

164
1-2
開始ページ
56
終了ページ
66
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.vetimm.2014.12.011
出版者・発行元
ELSEVIER SCIENCE BV

The aim of this study was to determine whether the egg-laying phase and estrogen affect the induction of cytotoxic cells in response to avian infectious bronchitis (IB) virus at early stage of infection in the oviduct. Attenuated IB virus (aIBV group) or its vehicle (control group) was introduced to the oviductal magnum lumen of White Leghorn hens in the laying and molting phase, as well as molting hens injected with estradiol benzoate (M-EB hens) or corn oil (M-oil hens). Oviductal isthmus and uterus were collected 24 h after injection. The frequency of CD8(+) and TCR gamma delta(+) T cells expression was examined by immunohistochemistry, followed by image analysis. The expression of the genes of toll-like receptor 7 (TLR7), natural killer cell receptor (BNK), cytotoxic substances (granzyme, perforin), and cytokines (CXCL12, CX3CL1, and IFN gamma) were examined by real-time polymerase chain reaction analysis. The frequency of CD8(+) and TCR gamma delta(+) T cells in the isthmus, and CD8(+) cells in the uterus was significantly higher in the aIBV group compared to the control group of laying and M-EB hens. The expression of all the genes examined in this study in the isthmus, and CX3CL1 and IFN gamma expression in the uterus was significantly higher in the aIBV group in the laying and M-EB hens. These results suggested that infection with IB virus causes an immune response involving the influx of cytotoxic cells and upregulation of cytokines in the isthmus and uterus at early stage of infection. This response was stronger during the laying phase compared to the molting phase, probably due to the effect of estrogen. (C) 2015 Elsevier B.V. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.vetimm.2014.12.011
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000352251000008&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.vetimm.2014.12.011
  • ISSN : 0165-2427
  • eISSN : 1873-2534
  • Web of Science ID : WOS:000352251000008

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