MISC

1997年2月

Establishment and characterization of a novel human cell line exhibiting both immunophenotypic markers of monocyte/macrophage and natural killer cell lineages from peripheral blood of a patient with atopic dermatitis

EXPERIMENTAL DERMATOLOGY
  • Y Hamamoto
  • ,
  • K Nagai
  • ,
  • M Muto
  • ,
  • K Nakamura
  • ,
  • H Yasui
  • ,
  • C Asagami

6
1
開始ページ
49
終了ページ
53
記述言語
英語
掲載種別
DOI
10.1111/j.1600-0625.1997.tb00145.x
出版者・発行元
MUNKSGAARD INT PUBL LTD

Large amounts of homogeneous cells are not always available for in vitro studies of inflammatory skin disorders. Here we demonstrate that a novel cell line, termed YAA, has been established from peripheral blood mononuclear cells, which were separated by the Ficoll method, of a patient with atopic dermatitis. YAA cells were grown in suspension culture. The cytochemical staining showed a positive reaction for a-naphthyl butyrate esterase, which was completely inhibited by sodium fluoride, but a negative result for periodic acid-Schiff, peroxidase and alkaline phosphatase. A large population of YAA cells exhibited phenotype of CD33 and CD56 but neither of CD2 nor CD3. The phorbol ester-stimulated YAA cells produced a considerable amount of tumor necrosis factor-alpha. These findings suggest that YAA might be a monocytoid line with an additional phenotype specific for natural killer cells.

リンク情報
DOI
https://doi.org/10.1111/j.1600-0625.1997.tb00145.x
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1997XK96800008&DestApp=WOS_CPL
ID情報
  • DOI : 10.1111/j.1600-0625.1997.tb00145.x
  • ISSN : 0906-6705
  • Web of Science ID : WOS:A1997XK96800008

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