1997年2月
Establishment and characterization of a novel human cell line exhibiting both immunophenotypic markers of monocyte/macrophage and natural killer cell lineages from peripheral blood of a patient with atopic dermatitis
EXPERIMENTAL DERMATOLOGY
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- 巻
- 6
- 号
- 1
- 開始ページ
- 49
- 終了ページ
- 53
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1111/j.1600-0625.1997.tb00145.x
- 出版者・発行元
- MUNKSGAARD INT PUBL LTD
Large amounts of homogeneous cells are not always available for in vitro studies of inflammatory skin disorders. Here we demonstrate that a novel cell line, termed YAA, has been established from peripheral blood mononuclear cells, which were separated by the Ficoll method, of a patient with atopic dermatitis. YAA cells were grown in suspension culture. The cytochemical staining showed a positive reaction for a-naphthyl butyrate esterase, which was completely inhibited by sodium fluoride, but a negative result for periodic acid-Schiff, peroxidase and alkaline phosphatase. A large population of YAA cells exhibited phenotype of CD33 and CD56 but neither of CD2 nor CD3. The phorbol ester-stimulated YAA cells produced a considerable amount of tumor necrosis factor-alpha. These findings suggest that YAA might be a monocytoid line with an additional phenotype specific for natural killer cells.
- リンク情報
- ID情報
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- DOI : 10.1111/j.1600-0625.1997.tb00145.x
- ISSN : 0906-6705
- Web of Science ID : WOS:A1997XK96800008