論文

査読有り 国際誌
2019年6月

Cytoplasmic R-peptide of murine leukemia virus envelope protein negatively regulates its interaction with the cell surface receptor.

Virology
  • Yoshinao Kubo
  • ,
  • Mai Izumida
  • ,
  • Kei Togawa
  • ,
  • Fengmin Zhang
  • ,
  • Hideki Hayashi

532
開始ページ
82
終了ページ
87
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.virol.2019.04.005

Cytoplasmic tails of envelope (Env) glycoproteins of many retroviruses inhibit their membrane fusion activity. The cytoplasmic 16-amino acid peptide of ecotropic murine leukemia virus (E-MLV) Env protein, called the R-peptide, also inhibits the membrane fusion activity of the Env protein. However, the molecular mechanism of the inhibition has not been elucidated yet. In this study, we found that R-peptide-containing Env protein of E-MLV binds to the cell surface receptor cationic amino acid transporter-1 (CAT-1) with weaker affinity than R-peptide-truncated Env protein. Consistent with this result, R-peptide-containing Env protein had less efficient inhibition of E-MLV vector infection than R-peptide-truncated Env protein. R-peptide truncation has been reported to induce conformational change in the surface subunit of E-MLV Env protein that interacts with the receptor. Taken together, our findings indicate that R-peptide truncation induces conformational change in the receptor-binding domain of the E-MLV Env protein and facilitates the Env-receptor interaction.

リンク情報
DOI
https://doi.org/10.1016/j.virol.2019.04.005
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31035110
ID情報
  • DOI : 10.1016/j.virol.2019.04.005
  • ISSN : 0042-6822
  • PubMed ID : 31035110

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