2015年7月
Crystal structure of a Xenopus laevis skin proto-type galectin, close to but distinct from galectin-1
GLYCOBIOLOGY
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- 巻
- 25
- 号
- 7
- 開始ページ
- 792
- 終了ページ
- 803
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1093/glycob/cwv020
- 出版者・発行元
- OXFORD UNIV PRESS INC
Xenopus laevis (African clawed frog) has two types of proto-type galectins that are similar to mammalian galectin-1 in amino acid sequence. One type, comprising xgalectin-Ia and -Ib, is regarded as being equivalent to galectin-1, and the other type, comprising xgalectin-Va and -Vb, is expected to be a unique galectin subgroup. The latter is considerably abundant in frog skin; however, its biological function remains unclear. We determined the crystal structures of two proto-type galectins, xgalectin-Ib and -Va. The structures showed that both galectins formed a mammalian galectin-1-like homodimer, and furthermore, xgalectin-Va formed a homotetramer. This tetramer structure has not been reported for other galectins. Gel filtration and other experiments indicated that xgalectin-Va was in a dimer-tetramer equilibrium in solution, and lactose binding enhanced the tetramer formation. The residues involved in the dimer-dimer association were conserved in xgalectin-Va and -Vb, and one of the Xenopus (Silurana) tropicalis proto-type galectins, but not in xgalectin-Ia and -Ib, and other galectin-1-equivalent proteins. Xgalectin-Va preferred Gal beta 1-3GalNAc and not Gal beta 1-4GlcNAc, while xgalectin-Ib preferred Gal beta 1-4GlcNAc as well as human galectin-1. Xgalectin-Va/Vb would have diverged from the galectin-1 group with accompanying acquisition of the higher oligomer formation and altered ligand selectivity.
- リンク情報
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- DOI
- https://doi.org/10.1093/glycob/cwv020
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/25804418
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000357522600011&DestApp=WOS_CPL
- URL
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84936741955&origin=inward
- ID情報
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- DOI : 10.1093/glycob/cwv020
- ISSN : 0959-6658
- eISSN : 1460-2423
- PubMed ID : 25804418
- SCOPUS ID : 84936741955
- Web of Science ID : WOS:000357522600011