MISC

2003年12月

A decay of gap junctions associated with ganglion cell differentiation during retinal regeneration of the adult newt

VISION RESEARCH
  • H Oi
  • ,
  • C Chiba
  • ,
  • T Saito

43
27
開始ページ
2847
終了ページ
2859
記述言語
英語
掲載種別
DOI
10.1016/j.visres.2003.07.006
出版者・発行元
PERGAMON-ELSEVIER SCIENCE LTD

Changes in the gap junctional coupling and maturation of voltage-activated Na(+) currents during regeneration of newt retinas were examined by whole-cell patch-clamping in slice preparations. Progenitor cells in regenerating retinas did not exhibit Na(+) currents but showed prominent electrical and tracer couplings. Cells identified by LY-fills were typically slender. Na(+) currents were detected in premature ganglion cells with round somata in the 'intermediate-II' regenerating retina. No electrical and tracer couplings were observed between these cells. Mature ganglion cells did not exhibit electrical coupling, but showed tracer coupling. On average, the maximum Na(+) current amplitude recorded from premature ganglion cells was roughly 2.5-fold smaller than that of mature ganglion cells. In addition, the activation threshold of the Na(+) current was nearly 11 mV more positive than that of mature cells. We provide morphological and physiological evidence showing that loss of gap junctions between progenitor cells is associated with ganglion cell differentiation during retinal regeneration and that new gap junctions are recreated between mature ganglion cells. Also we provide evidence suggesting that the loss of gap junctions correlates with the appearance of voltage-activated Na(+) currents in ganglion cells. (C) 2003 Elsevier Ltd. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.visres.2003.07.006
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000186297900001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.visres.2003.07.006
  • ISSN : 0042-6989
  • Web of Science ID : WOS:000186297900001

エクスポート
BibTeX RIS