1997年3月
Localization of a DNA topoisomerase II to mitochondria in Dictyostelium discoideum: Deletion mutant analysis and mitochondrial targeting signal presequence
JOURNAL OF PLANT RESEARCH
- ,
- ,
- ,
- ,
- 巻
- 110
- 号
- 1097
- 開始ページ
- 65
- 終了ページ
- 75
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- 出版者・発行元
- BOTANICAL SOC JAPAN
DNA topoisomerase II of Dictyostelium discoideum (TopA), the gene (topA) encoding which we cloned, was shown to have an additional N-terminal region which contains a putative mitochondrial targeting signal presequence. We constructed overexpression mutants which expressed the wild-type or the N-terminally deleted enzyme, and examined its localization by immunofluorescence microscopy and proteinase K digestion experiment. These experiments revealed that the enzyme is located in the mitochondria by virtue of the additional N-terminal region. Furthermore, in the cell extract depleted the enzyme by immunoprecipitation, nuclear DNA topoisomerase If activity was not decreased. These results confirmed that TopA is located in the mitochondria, even though its amino acid sequence is highly similar to those of nuclear type topoisomerase II of other organisms. Thus, this report is the first to establish the location of the mitochondrial targeting signal presequence in DNA topoisomerase II and in proteins of D. discoideum directly by analyzing deletion mutants.
- リンク情報
- ID情報
-
- ISSN : 0918-9440
- Web of Science ID : WOS:A1997WX67600009