2019年10月1日
Endophytic fungal flora in the living leaves of Fraxinus excelsior healthily growing in Hokkaido, Japan
Asian Mycological Congress 2019 (AMC 2019)
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- 開催年月日
- 2019年10月1日 - 2019年10月4日
- 記述言語
- 英語
- 会議種別
- ポスター発表
- 開催地
- Tsu, Mie,
Purpose: Ash dieback caused by Hymenoscyphus fraxineus (Helotiales) is threatening Fraxinus species
in Europe. Kato (2018) showed that H. fraxineus endophytically inhabits several ashes growing in
Hokkaido Pref., Japan by detection of fungal DNA. Among them, F. excelsior (European ash) that is
known as a susceptible species, but healthily growing in the Sapporo campus of Hokkaido Univ. was
included. Other fungi coexisting in the same tissues may affect to H. fraxineus and function as one of
the biological factors to control the disease. In this study, assemblages of endophytes in healthy leaves
of the F. excelsior were investigated toward exploration of biological agents for ash dieback control.
Methods: Assemblages of endophytes in leaflets and rachises of the healthily growing F. excelsior were
explored by a culture-based method and a metagenome analysis. Fraxinus pennsylvanica (Green ash)
and F. mandshurica (Manchurian ash) in the site were examined by a culture-based method as well.
Results and Conclusion: 22 species of 15 genera in 14 families were isolated from the surface-sterilized
leaves of F. excelsior. Aureobasidium pullulans, Diaporthe spp. and Colletotrichum spp. were isolated in
high frequency. Talaromyces marneffei was isolated only from F. excelsior. In the metagenome analysis,
186 species were recognized in the leaves that were processed by ultrasonic-washing with dioctyl
sodium sulfosuccinate (Aerosol OT). The most frequently detected species was A. pullulans, followed
by Papiliotrema flavescens. Such fungi detected in high frequency may dominate the endophytic flora.
Further studies are required to explore the antagonisms of those fungi against H. fraxineus.
in Europe. Kato (2018) showed that H. fraxineus endophytically inhabits several ashes growing in
Hokkaido Pref., Japan by detection of fungal DNA. Among them, F. excelsior (European ash) that is
known as a susceptible species, but healthily growing in the Sapporo campus of Hokkaido Univ. was
included. Other fungi coexisting in the same tissues may affect to H. fraxineus and function as one of
the biological factors to control the disease. In this study, assemblages of endophytes in healthy leaves
of the F. excelsior were investigated toward exploration of biological agents for ash dieback control.
Methods: Assemblages of endophytes in leaflets and rachises of the healthily growing F. excelsior were
explored by a culture-based method and a metagenome analysis. Fraxinus pennsylvanica (Green ash)
and F. mandshurica (Manchurian ash) in the site were examined by a culture-based method as well.
Results and Conclusion: 22 species of 15 genera in 14 families were isolated from the surface-sterilized
leaves of F. excelsior. Aureobasidium pullulans, Diaporthe spp. and Colletotrichum spp. were isolated in
high frequency. Talaromyces marneffei was isolated only from F. excelsior. In the metagenome analysis,
186 species were recognized in the leaves that were processed by ultrasonic-washing with dioctyl
sodium sulfosuccinate (Aerosol OT). The most frequently detected species was A. pullulans, followed
by Papiliotrema flavescens. Such fungi detected in high frequency may dominate the endophytic flora.
Further studies are required to explore the antagonisms of those fungi against H. fraxineus.