MISC

2010年

Expression of Phosphatidylserine-Specific Phospholipase A(1) mRNA in Human THP-1-Derived Macrophages

CELL TRANSPLANTATION
  • Hiroyuki Hosono
  • ,
  • Masato Homma
  • ,
  • Yoko Ogasawara
  • ,
  • Kumiko Makide
  • ,
  • Junken Aoki
  • ,
  • Hideaki Niwata
  • ,
  • Machiko Watanabe
  • ,
  • Keizo Inoue
  • ,
  • Nobuhiro Ohkohchi
  • ,
  • Yukinao Kohda

19
6-7
開始ページ
759
終了ページ
764
記述言語
英語
掲載種別
DOI
10.3727/096368910X508861
出版者・発行元
COGNIZANT COMMUNICATION CORP

The expression of phosphatidylserine-specific phospholipase A(1) (PS-PLA(1)) is most upregulated in the genes of peripheral blood cells from chronic rejection model rats bearing long-term surviving cardiac allografts. The expression profile of PS-PLA(1) in peripheral blood cells responsible for the immune response may indicate a possible biological marker for rejection episodes. In this study, PS-PLA(1) mRNA expression was examined in human THP-1-derived macrophages. The effects of several immunosuppressive agents on this expression were also examined in in vitro experiments. A real-time RT-PCR analysis revealed that PS-PLA(1) mRNA expression was found in human THP-1-derived macrophages. This expression was enhanced in the cells stimulated with lipopolysaccharide (LPS), a toll-like receptor (TLR) 4 ligand. Other TLR ligands (TLR2, 3, 5, 7, and 9) did not show a significant induction of PS-PLA(1) mRNA. The time course of the mRNA expression profiles was different between PS-PLA(1) and tumor necrosis factor-alpha (TNF-alpha), which showed a maximal expression at 12 and I h after LPS stimulation, respectively. Among the observed immunosuppressive agents, corticosteroids, prednisolone, 6 alpha-methylprednisolone, dexamethasone, and beclomethasone inhibited PS-PLA(1) expression with half-maximal inhibitory concentrations less than 3.0 nM, while methotrexate, cyclosporine A, tacrolimus, 6-mercaptopurine, and mycophenoic acid showed either a weak or moderate inhibition. These results suggest that the expression of PS-PLA(1) mRNA in THP-1-derived macrophages is activated via TLR4 and it is inhibited by corticosteroids, which are used at high dosages to suppress chronic allograft rejection.

リンク情報
DOI
https://doi.org/10.3727/096368910X508861
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000282860500014&DestApp=WOS_CPL
ID情報
  • DOI : 10.3727/096368910X508861
  • ISSN : 0963-6897
  • Web of Science ID : WOS:000282860500014

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