2008年5月
Protein kinase CβI interacts with the β1-adrenergic signaling pathway to attenuate lipolysis in rat adipocytes
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
- 巻
- 1781
- 号
- 5
- 開始ページ
- 277
- 終了ページ
- 281
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/j.bbalip.2008.03.007
We have shown previously that insulin attenuates β1-adrenergic receptor (β1-AR)-mediated lipolysis via activation of protein kinase C (PKC) in rat adipocytes. This antilipolysis persists after removal of insulin and is independent of the phosphodiesterase 3B activity, and phorbol 12-myristate 13-acetate (PMA) could substitute for insulin to produce the same effect. Here, we attempted to identify the PKC isoform responsible for antilipolysis. Isolated adipocytes were treated with high and low concentrations of PMA for up to 6 h to degrade specific PKC isoforms. In the PMA-treated cells, the downregulation profiles of PKC isoforms α and βI, but not βII, δ, e{open}, or ζ, correlated well with a decrease of lipolysis-attenuating effect of PMA. After rats fasted for 24 h, adipocyte expression of PKC isoform α increased, while expression of PKCδ decreased. Fasting did not change the potency of PMA to attenuate lipolysis, however. The lipolysis-attenuating effect of PMA was blocked by the PKCβI/βII inhibitor LY 333531, but not by the PKCβII inhibitor CGP 53353 or the PKCδ inhibitor rottlerin. These data suggest that PKCβI interacts with β1-AR signaling and attenuates lipolysis in rat adipocytes. © 2008 Elsevier B.V. All rights reserved.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.bbalip.2008.03.007
- ISSN : 1388-1981
- PubMed ID : 18440322
- SCOPUS ID : 43149096898