2006年3月
Protein kinase C attenuates beta-adrenergic receptor-mediated lipolysis, probably through inhibition of the beta(1)-adrenergic receptor system
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
- 巻
- 447
- 号
- 1
- 開始ページ
- 1
- 終了ページ
- 10
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/j.abb.2006.01.011
- 出版者・発行元
- ELSEVIER SCIENCE INC
Lipolysis in rat white adipocytes is stimulated by P-adrenergic agonists. Phorbol 12-myristate 13-acetate (PMA) attenuated the receptor-mediated lipolysis by causing a shift of the dose-response curve to the higher concentrations of norepinephrine and isoproterenol. Although the adipocytes possess beta(-)(1), beta(-)(2), and beta(3)-adrenergic receptor Subtypes, the effect of PMA was observed only when a beta(1)-agonist (dobutamine) was used. No lipolysis-attenuating effect of PMA was found when cells were exposed to a beta(2)-agonist (procaterol) and beta(3)-agonists (BRL 37344 and CL 316243), or to forskolin and 8-brorno cAMP. CGP 20712A (PI-antagonist) efficiently inhibited lipolysis by norepinephrine, isoproterenol, and dobutamine, but did not affect lipolysis by the beta(2)- and beta(3)-agonists. ICI 118551 (beta(2)-antagonist) had no significant effect on lipolysis by the beta-agonists examined. CGP 20712A abolished the lipolysis-attenuating effect of PMA, but ICI 118551 did not. The protein kinase C (PKC) inhibitors. GF 109203X or Go 6976, suppressed the effect of PMA. Pretreatment of adipocytes with PMA for 6h caused downregulation of conventional and novel PKCs in association with a decrease in the lipolysis-attenuating effect of PMA. These results indicate that conventional and novel PKCs attenuate lipolysis mediated by beta-adrenergic receptors, probably through inhibition of the beta(1)-adrenergic receptor system. (c) 2006 Elsevier Inc. All rights reserved.
- リンク情報
-
- DOI
- https://doi.org/10.1016/j.abb.2006.01.011
- CiNii Articles
- http://ci.nii.ac.jp/naid/80019273391
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/16500613
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000236052300001&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1016/j.abb.2006.01.011
- ISSN : 0003-9861
- CiNii Articles ID : 80019273391
- PubMed ID : 16500613
- Web of Science ID : WOS:000236052300001