1999年5月
Oxidative DNA damage induced by aminoacetone, an amino acid metabolite
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
- ,
- ,
- ,
- 巻
- 365
- 号
- 1
- 開始ページ
- 62
- 終了ページ
- 70
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1006/abbi.1999.1161
- 出版者・発行元
- ELSEVIER SCIENCE INC
We investigated DNA damage induced by aminoacetone, a metabolite of threonine and glycine. Pulsed-field gel electrophoresis revealed that aminoacetone caused cellular DNA cleavage. Aminoacetone increased the amount of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in human cultured cells in a dose-dependent manner. The Formation of 8-oxodG in calf thymus DNA increased due to aminoacetone only in the presence of Cu(II). DNA ladder formation was observed at higher concentrations of aminoacetone than those causing DNA cleavage. Flow cytometry showed that aminoacetone enhanced the generation of hydrogen peroxide (H(2)O(2)) in cultured cells. Aminoacetone caused damage to (32)P-5'-end-labeled DNA fragments, obtained from the human c-Ha-ras-1 and p53 genes, at cytosine and thymine residues in the presence of Cu(II). Catalase and bathocuproine inhibited DNA damage, suggesting that H(2)O(2) and Cu(I) were involved. Analysis of the products generated from aminoacetone revealed that aminoacetone underwent Cu(II)-mediated autoxidation in two different pathways: the major pathway in which methylglyoxal and NH; are generated and the minor pathway in which 2,5-dimethylpyrazine is formed through condensation of two molecules of aminoacetone. These findings suggest that H(2)O(2) generated by the autoxidation of aminoacetone reacts with Cu(I) to form reactive species capable of causing oxidative DNA damage. (C) 1999 Academic Press.
- リンク情報
-
- DOI
- https://doi.org/10.1006/abbi.1999.1161
- CiNii Articles
- http://ci.nii.ac.jp/naid/30017926667
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/10222039
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000080059600008&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1006/abbi.1999.1161
- ISSN : 0003-9861
- CiNii Articles ID : 30017926667
- PubMed ID : 10222039
- Web of Science ID : WOS:000080059600008