To establish the method of cultivation of Polygala tenuifolia, one of the source plants for the crude drug "Onji", the factors influencing seed germination such as temperature, light, seeding depth and seed storage conditions were investigated to give the following results. The seeds of P. tenuifolia kept with wet sand at 4℃ for 1 year germinated at 15℃ and 25℃ in a Petri dish, but the germination rate was considerably lower when they were germinated in the dark.In the field test, the germination rate of the seeds stored at room temperature in the dark also dropped when the seeds were covered with soil. These results indicated seeds of P. tenuifolia required light for germination.The effects of the bag materials, temperatures and durations used for seed storage on germination were assayed to show that the germination rates were influenced by the duration of storage and storage temperature. Furthermore, when stored at room temperature, the seeds stored in a polyvinyl bag germinated at a higher rate compared with those stored in a paper bag. So, it is reasonable to consider that the seed germination rate of this species is controlled by the seed dormancy with loss of water and its break with low temperature. The recommended points for cultivation of P. tenuifolia may be summerized as follows; (1) For one year storage, the seeds should be stored with wet sand. Seeds without wet sand are to be keep at room temperature in a polyvinyl bag. For two year storage, seeds in the polyvinyl bag must be kept at a low temperature (4 or -20℃).(2) After sowing, press the sowed soil surface softly instead of covering the seeds with soil.