MISC

2000年

Osmotic and non - osmotic induction of somatic embryogenesis by sucrose at high concentrations in Dauern carota L

Plant Biotechnology
  • Kouji Satoh
  • ,
  • Hisako Ooka
  • ,
  • Atsuko Wakai
  • ,
  • Yoshinori Takahara
  • ,
  • Koji Yamamoto

17
2
開始ページ
155
終了ページ
158
記述言語
英語
掲載種別
DOI
10.5511/plantbiotechnology.17.155
出版者・発行元
Japanese Society for Plant Cell and Molecular Biology

In the detached cotyledons of carrot, Daucus carota L., somatic embryogenesis was induced by 2week treatment with sucrose at 0.29-0.73 M both in the epidermal cells (type-1) and the callus-like cells generated at the cut end of the cotyledons (type-2). Type-2 embryos were produced predominantly, after the treatment with 0.29-0.44 M sucrose and type-1 embryos after treatment with 0.58-0.73 M sucrose. Sucrose and glucose were more effective than mannitol and sorbitol for the induction of somatic embryogenesis. Furthermore, both type - 1 and type - 2 embryos were induced by sucrose and glucose, but only type-2 embryos by mannitol and sorbitol. These results suggest that sucrose does not work as a mere osmoticum.

リンク情報
DOI
https://doi.org/10.5511/plantbiotechnology.17.155
CiNii Articles
http://ci.nii.ac.jp/naid/10010146490

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